up-regulation of plasma membrane h+-atpase under salt stress may enable aeluropus littoralis to cope with stress

Authors

hosna olfatmiri

abbas alemzadeh

zahra zakipour

abstract

plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence of the gene encoding plasma membrane h+-atpase in aeluropus littoralis to amplify a 259 bp fragment from the target gene by pcr. a gene encoding actin was used as reference gene to normalize the expression level of the target gene. a pair of specific primers was designed to amplify a 157 bp fragment from the actin gene by pcr. plants were treated with different concentrations of nacl, 0, 50, 100, 150, 200, 250, 500 and 1000 mm, for two days. our results showed that the expression level of the plasma membrane h+-atpase gene increased dramatically at 500 mm and then decreased with increasing concentrations of nacl. the results also indicated that the leaves of plants, were treated with high concentrations of nacl changed morphologically, but those grown under low concentrations of nacl as well as the control plants did not show morphological changes in their leaves. our results suggest a relation between morphological changes of treated plants and the expression level of the plasma membrane h+-atpase gene in aeluropus littoralis.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Up-regulation of plasma membrane H+-ATPase under salt stress may enable Aeluropus littoralis to cope with stress

Plasma membrane H+-ATPase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. To study the effect of NaCl on the expression pattern of a gene encoding the plasma membrane H+-ATPase, an experiment was carried out in a completely random design with three replications. A pair of specific primers was designed based on the sequence o...

full text

Up-regulation of plasma membrane H+-ATPase under salt stress may enable Aeluropus littoralis to cope with stress

Most plants encounter stress such as drought and salinity that adversely affect growth, development and crop productivity. The expression of the gene glutathione-s-transferases (GST) extends throughout various protective mechanisms in plants and allows them to adapt to unfavorable environmental conditions. GSTF1 (the first phi GSTFs class) gene expression patterns in the wheat cultivars Mahuti ...

full text

Heavy metal regulation of plasma membrane H+-ATPase gene expression in halophyte Aeluropus littoralis

The present study was conducted to find the effect of three heavy metals, Ag, Hg and Pb on the expression level of a gene encoding plasma membrane H+-ATPase in Aeluropus littoralis. The experiment was laid out in a completely random design with three replications. The expression of the main gene was normalized to the expression of the housekeeping gene actin. Two 259 and 187 bp fragments were a...

full text

heavy metal regulation of plasma membrane h+-atpase gene expression in halophyte aeluropus littoralis

the present study was conducted to find the effect of three heavy metals, ag, hg and pb on the expression level of a gene encoding plasma membrane h+-atpase in aeluropus littoralis. the experiment was laid out in a completely random design with three replications. the expression of the main gene was normalized to the expression of the housekeeping gene actin. two 259 and 187 bp fragments were a...

full text

Isolation, molecular cloning and expression analysis of Aeluropus littoralis Monodehydroascorbate reductase (MDHAR) gene under salt stress

Plants are naturally exposed to frequent changes in environmental conditions such as salt stress affecting their growth and development, and consequently a considerable reduction in total productivity. Monodehydroascorbate reductase (MDHAR), a key enzyme in ascorbate-glutathione cycle, serves as an important antioxidative enzyme in scavenging of reactive oxygen spices (ROS). In this study, ...

full text

Morphological and anatomical changes in stems of Aeluropus littoralis under salt stress

Salinity is one of the most important agricultural issues causing considerable yield reduction in agricultural crops. The main adverse effects of salinity are due to excess amount of sodium ions that is toxic to plant cells. Most halophytes are equipped with defense mechanisms enabling them to tolerate the salty habitats. Among grass plants, Aeluropus littoralis is a known monocots hal...

full text

My Resources

Save resource for easier access later


Journal title:
molecular biology research communications

Publisher: shiraz university press

ISSN 2322-181X

volume 3

issue 1 2014

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023